Ezio also makes an appearance as a playable character in the fighting game Soulcalibur V.Ezio is an ancestor of Desmond Miles, the protagonist of most of the series' backstory, who experiences Ezio's life through a device unlocking hidden memories inside his DNA.Along the way, Ezio saves many Assassins who help him, including Luis de Santángel and Raphael Sanchez.
These allies train Ezio into an Assassin and guide him on his quest.
Giovanni attempts to present the evidence to Uberto Alberti, the Gonfaloniere of Florence.
Uberto, however, is revealed to also be conspirator and orders for the Auditore family's arrest, blaming them for the plot.
Ezio has to save Christopher Columbus and kill Tomás de Torquemada to end the Templar threat.
Ezio must also assassinate some other important targets such as Gasper Martinez, Pedro Llorente, a Templar spy, and Juan de Marillo.During the events of Assassin's Creed II: Discovery, which plays during Assassin's Creed II, Ezio travels to Spain in 1490 to free his fellow Assassins, who have been imprisoned under the guise of the Spanish Inquisition.